Title:
【発明の名称】担体およびオリゴヌクレオチドの合成
Document Type and Number:
Japanese Patent JP2548112
Kind Code:
B2
Abstract:
A versatile polymeric support system for the synthesis of oligonucleotides featuring a universal primer which allows chain elongation, in either the 3' or 5' direction, with any currently available DNA or RNA synthesis method, by a process which utilizes oxidatively cleaved primers to facilitate chain elongation and release. The support system is capable of withstanding mildly basic and acidic reaction conditions, while still permitting a convenient and quantitative release, either before or after removal of protecting groups from reactive groups, of synthesized oligonucleotides from a single polymeric support. Removal of the protecting groups before cleavage of the oligomer from the support permits the use of the immobilized oligomer as an affinity hybridization support for both isolation and detecting complementary polynucleic acids.
Inventors:
ARNOLD LYLE J JR (US)
Application Number:
JP50332784A
Publication Date:
October 30, 1996
Filing Date:
August 21, 1984
Export Citation:
Assignee:
MOLECULAR BIOSYSTEMS INC (US)
International Classes:
B01J20/281; G01N33/58; B01J20/285; C07H19/06; C07H19/16; C07H21/00; C07H21/04; C08F8/00; C12N15/09; C12Q1/68; G01N30/88; (IPC1-7): C07H21/00
Attorney, Agent or Firm:
Shusaku Yamamoto