Title:
【発明の名称】負の選択マーカーおよびサイトカインをコード化する遺伝子を用いる腫瘍細胞の遺伝子転換による腫瘍の治療
Document Type and Number:
Japanese Patent JPH09504518
Kind Code:
A
Abstract:
A method of treating a tumor in a host which comprises administering to the host at least one expression vehicle including a first nucleic acid sequence encoding an agent which renders the tumor cells sensitive to an interaction agent, and a second nucleic acid sequence which encodes an agent which provides for the inhibition, prevention, or destruction of the growth of the tumor cells, wherein such agent is not an agent which renders tumor cells sensitive to an interaction agent. The tumor then is treated with the interaction agent. Preferably, the first nucleic acid sequence encodes a negative selective marker (such as Herpes Simplex Virus thymidine kinase), and the second nucleic acid sequence encodes a cytokine (such as Interleukin-2), and the first and second nucleic acid sequences are contained in first and second viral vectors, each of which is contained in a first producer cell line and in a second producer cell line, respectively. The producer cell lines are administered to the tumor, whereby viral particles generated by the producer cell lines infect the tumor cells. Upon administration of an interaction agent, such as ganciclovir, to the host, the tumor cells are killed.
Inventors:
Culver, Kennes, W.
Link, Charles, Jay., Junior
Blaise, Michael, Earl.
Link, Charles, Jay., Junior
Blaise, Michael, Earl.
Application Number:
JP51098895A
Publication Date:
May 06, 1997
Filing Date:
October 04, 1994
Export Citation:
Assignee:
THE UNITED STATES OF AMERICA
International Classes:
C12N15/09; A61K31/52; A61K31/70; A61K35/76; A61K38/16; A61K39/00; A61K48/00; A61P35/00; C07H21/04; C07K14/55; C12N5/10; A61K38/00; (IPC1-7): A61K48/00; A61K31/52; A61K31/70; A61K35/76; A61K38/16; C07H21/04; C12N5/10; C12N15/09
Attorney, Agent or Firm:
Hiroyuki Niwa