PURPOSE: To determine an L-amino acid quickly and accurately at low cost, by carrying out the decarboxylation reaction of an L-amino acid to be determined using an enzymatic system in a mold of microorganism, by measuring formed carbon dioxide gas by a quadroupole type mass spectrometer.
CONSTITUTION: The measurement probe 6 is constructed by attaching a mold of microorganism having a decarboxylase activity on an L-amino acid to be analyzed or the enzyme 2 derived from the mold to the permeable membrane 1 of a probe for gas introduction, having a tip covered with the gap permeable film 4, connected to a quadrupole type mass spectrometer. This measurement probe 6 is under anaerobic conditions brought into contact with a specimen solution to be measured containing an L-amino acid to be determined, the decarboxylation reaction of the L-amino caid is carried out, the concentration of the substrate is obtained by utilizong the proportional relationship between the concentration of the substrate and the output electric current of the quadrupole type mass spectrometer corresponding to evolved carbon dioxide gas, so that the desired L-amino acid is determined.
| WO/2021/067781 | SPECIMEN IMAGING SYSTEMS AND METHODS |
| WO/2019/229451 | TRANSFER LINE, GCMS ARRANGEMENT AND MOUNTING ASSEMBLY |
| JP5421468 | Pulsed mass calibration in time-of-flight mass spectrometry |
HIKIMA MOTOHIKO
OBANA HARUO
GOTOU YUUJI
MORITA YASUTSUGU
SUZUKI TSUGUO
| JPS5598348A | 1980-07-26 |