Title:
【発明の名称】ニトリルヒドラターゼ活性を有するポリペプチドをコードする遺伝子DNA、これを含有する形質転換体
Document Type and Number:
Japanese Patent JP3162091
Kind Code:
B2
Abstract:
DNA fragments (H) and (L), encoding polypeptides having nitrile hydratase activity, are new. (H) and (L) encode alpha(H), beta(H) and alpha(L) and beta(L) polypeptide subunits respectively, having sequences defined in the specification. Also claimed are: (1) a recombinant DNA (R) comprising (H) or (L); (2) a transformant transformed with (R); (3) prodn. of nitrile hydratase by culturing the transformant of (2) and recovering the enzyme from the culture; (4) prodn. of amides by hydrating nitriles using the hydratase obtd. in (3); and (5) prodn. of amides by culturing the transformant of (2), and hydrating nitriles to amides using the resultant culture, isolated bacterial cells, treated matter or fixed material. Pref. (H) and (L) have DNA sequences defined in the specification.
Inventors:
Teruhiko Beppu
Hideaki Yamada
Makoto Nishiyama
Tohru Nagasawa
Sueharu Horinouchi
Hideaki Yamada
Makoto Nishiyama
Tohru Nagasawa
Sueharu Horinouchi
Application Number:
JP3323691A
Publication Date:
April 25, 2001
Filing Date:
February 27, 1991
Export Citation:
Assignee:
Teruhiko Beppu
Hideaki Yamada
Hideaki Yamada
International Classes:
C07H21/04; C12N1/21; C12N9/34; C12N9/78; C12N15/53; C12N15/09; C12N15/31; C12N15/55; C12N15/60; C12P13/02; C12R1/01; C12R1/19; C12N; (IPC1-7): C12N15/09; C12N1/21; C12P13/02; C12R1:19
Other References:
Ｂｉｏｃｈｅｍ．Ｂｉｏｐｈｙｓ．Ｒｅｓ．Ｃｏｍｍｕｎ．，１５５（２）ｐｐ．１００８−１０１６（１９８８）
Ｅｕｒ．Ｊ．Ｂｉｏｃｈｅｍ．，１８１（３），ｐｐ．５６３−５７０（１９８９）
Ｅｕｒ．Ｊ．Ｂｉｏｃｈｅｍ．，１８１（３），ｐｐ．５６３−５７０（１９８９）
Attorney, Agent or Firm:
Yusuke Hiraki (2 outside)