Title:
【発明の名称】RNA依存性DNAプライミング及び逆転写酵素活性を有するヘパドナウイルスポリメラーゼ遺伝子生成物並びにその活性を測定する方法
Document Type and Number:
Japanese Patent JPH08503846
Kind Code:
A
Abstract:
An enzymatically active composition of matter, comprising an extracellular combination of a substantially virus-free hepadnavirus polymerase gene product and an RNA template for hepadnavirus minus strand DNA synthesis, is provided. The composition possesses a DNA priming activity and a reverse transcriptase activity. The hepadnavirus polymerase gene product is produced by in vitro expression of a hepadnavirus pol gene. The enzymatically active composition of matter is used in rapid in vitro assays to screen potential anti-hepadnaviral agents. Assays for various functions of the hepadnavirus DNA polymerase are provided, including: (1) binding of the polymerase gene product to hepadnavirus pre-genomic RNA; (2) DNA priming activity of the polymerase gene product; and (3) reverse transcriptase activity of the polymerase gene product.
Inventors:
Seeger Christoph
Application Number:
JP50734594A
Publication Date:
April 30, 1996
Filing Date:
August 27, 1993
Export Citation:
Assignee:
Fox Chase Cancer Center
International Classes:
C12N9/12; C12N15/09; C12N15/54; C12Q1/48; C12Q1/68; C12Q1/70; G01N33/573; G01N33/58; (IPC1-7): C12N9/12; C12N15/09; C12Q1/48; C12Q1/68; C12Q1/70; G01N33/573; G01N33/58
Attorney, Agent or Firm:
Ariga Gunichiro