Title:
【発明の名称】高度の発現性を持つ宿主細胞系からの、生物学的活性の高いインシュリン様成長因子Iの製造法
Document Type and Number:
Japanese Patent JPH04501514
Kind Code:
A
Abstract:
A novel method for refolding reduced IGF-I is provided in accordance with the present invention. The method is carried out by providing additional positive charge at the amino terminal end of the IGF-I molecule by the presence of a short leader sequence. The additional positive charge on the met-end of the IGF-I molecule has been found to enable recombinant IGF-I to refold simply by stirring solubilized inclusion body protein for between 2-16 hours, or overnight. The present invention also provides a novel fusion protein intermediate of IGF-I attached to the positively charged leader sequence.
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Inventors:
Miller, The Ames
Shea, Philip Kay
Tsai, Larry Bee
Shea, Philip Kay
Tsai, Larry Bee
Application Number:
JP51339190A
Publication Date:
March 19, 1992
Filing Date:
August 21, 1990
Export Citation:
Assignee:
Amjien Incorporated
International Classes:
A61K38/27; C07K14/00; C07K14/575; C07K14/65; C07K19/00; C12N15/62; C12N15/09; C12P21/02; C12R1/19; (IPC1-7): A61K37/36; C07K7/10; C07K99/00; C12N15/18; C12N15/62; C12P21/02
Attorney, Agent or Firm:
Yoshio Kawaguchi (4 outside)