Title:
【発明の名称】被験サンプル中の複数分析物の時差式検出方法
Document Type and Number:
Japanese Patent JPH10511460
Kind Code:
A
Abstract:
The instant invention provides immunoassay methods whereby the presence or amount of multiple analytes that may be present in a test sample can be detected. According to one embodiment, the method comprises the steps of: (a) contacting a test sample with a common capture reagent for a time and under conditions sufficient to form capture reagent/analyte complexes wherein the common capture reagent includes one or more specific binding members that immobilize at least two analytes that may be present in said test sample; (b) contacting the capture reagent/analyte complexes with at least two indicator reagents for a time and under conditions sufficient to form capture reagent/analyte/indicator reagent complexes; and (c) detecting at least two measurable signals as a measure of the presence or amount of the analytes in the test sample. Indicator reagents which are employed in the above embodiment can comprise detectable moieties from at least two distinct detectable moiety classes. The invention also applies to detecting multiple nucleic acid sequences which may be present in a test sample.
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Inventors:
Carril, Omar S
Hanryi, Careers A
Hanryi, Careers A
Application Number:
JP51992195A
Publication Date:
November 04, 1998
Filing Date:
December 18, 1995
Export Citation:
Assignee:
Abbot Laboratories
International Classes:
C12Q1/68; G01N33/543; G01N33/542; (IPC1-7): G01N33/543; G01N33/543
Attorney, Agent or Firm:
Yoshio Kawaguchi (2 outside)