Title:
【発明の名称】抗体の安定性を改良するための方法
Document Type and Number:
Japanese Patent JP3103116
Kind Code:
B2
Abstract:
PCT No. PCT/EP95/02626 Sec. 371 Date Jan. 14, 1997 Sec. 102(e) Date Jan. 14, 1997 PCT Filed Jul. 5, 1995 PCT Pub. No. WO96/02574 PCT Pub. Date Feb. 1, 1996A process for improving the stability of an antibody or fragment thereof is disclosed. A gene encoding for an amino acid sequence of a variable domain of an antibody or fragment thereof to be modified is provided, and the amino acid sequence of the gene is compared with one of consensus tables 1-6. At least one codon of each pair of codons in the gene, which pair together code for disulfide bridge-forming cysteines, are modified so that all disulfide bridges present in the antibody as produced in a eukaryotic cell are absent from the modified antibody as produced in the method. At least one additional codon in the gene which codes for an amino acid other than a disulfide bridge-forming cysteine is also modified. A prokaryotic microorganism is transformed with the modified gene and additional variable domain DNA, and the modified antibody or fragment thereof is expressed in the prokaryotic microorganism.
Inventors:
Styp, Boris
Steinbacher, Stefan
Steinbacher, Stefan
Application Number:
JP50464596A
Publication Date:
October 23, 2000
Filing Date:
July 06, 1995
Export Citation:
Assignee:
Roche Diagnostics Gesellschaft Mitt Beschlenktel Haftung
International Classes:
A61K39/395; C07H21/04; C07K16/00; C07K19/00; C12N1/21; C12N15/09; C12P21/08; G01N33/531; A61K38/00; C12R1/19; (IPC1-7): C12N15/09; C07K16/00; C07K19/00; C12P21/08
Other References:
【文献】国際公開93/6217(WO,A1)
Attorney, Agent or Firm:
Takashi Ishida (2 outside)