Title:
【発明の名称】転写活性のあるDNA断片を製造する方法
Document Type and Number:
Japanese Patent JP2002539800
Kind Code:
A
Abstract:
A method for producing transcriptionally active DNA molecules, comprising (PCR) amplification of said DNA molecule in the presence of a first DNA fragment (F1), second DNA fragment (F2), first primer (P1), a second primer (P2), a third primer (P3), and a fourth primer (P4) wherein: F1 comprises a promoter sequence; F2 comprises a terminator sequence; P1 is complementary to the 5' end of F1; P2 is complementary to the 5' end of F2; P3 comprises a first region complementary to the 3' end of F1 and a second region complementary to the 5' end of said DNA molecule; P4 comprises a first region complementary to the 3' end of F2 and a second region complementary to the 3' end of said DNA molecule.
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Inventors:
Liang, Xiao
Fergner, Philip Elle.
Fergner, Philip Elle.
Application Number:
JP2000606773A
Publication Date:
November 26, 2002
Filing Date:
March 23, 2000
Export Citation:
Assignee:
Gene Therapy Systems, Inc.
International Classes:
C12N15/09; C12N15/10; C12N15/66; C12N15/85; C12Q1/68; C12Q1/686; (IPC1-7): C12N15/09; C12Q1/68
Attorney, Agent or Firm:
Tsutomu Toyama (4 others)