Title:
【発明の名称】ヒト組換えデコリンの精製方法およびグアニジウムイオンの検出方法
Document Type and Number:
Japanese Patent JPH10506523
Kind Code:
A
Abstract:
The present invention is directed to a process for the production of substantially pure human recombinant decorin which involves the combination of three separate stages characterized by contacting decorin-containing cell culture medium with (1) a first strong anionic exchange resin; then with (2) a hydrophobic interactive chromatographic resin; and finally with (3) a second strong anionic exchange resin. By using a combination of steps, and certain reagents, in particular a 2.4 to 3 molar GuHCl solution to elute decorin from a hydrophobic interactive column, the process of this invention provides a more convenient and reproducible process for purifying human recombinant decorin. The invention also provides a process for detecting the presence of guanidinium ions in a sample solution. The detection process involves contacting a sample solution suspected of containing guanidinium ions with a cation exchange resin and eluting the guanidinium ions present in tire sample solution with an aqueous buffer solution having a pH of about 1.5 to about 2. This is followed by contacting the eluant with a cation suppressor columns and simultaneously flowing a suppressor regenerate solution in the opposite direction on the opposite side of the permeable membrane of the column, and finally, detecting the presence of guanidinium ions in the eluant from the ion exchange column which was contacted with the suppressor column by use of a conductivity detector.
Inventors:
Craig, William S.
Harper, John Earl.
Hernandez, Sam Dee.
Costel, Paul Jay.
Parker, Jonathan Earl.
Bedvic, Thomas S.
Harper, John Earl.
Hernandez, Sam Dee.
Costel, Paul Jay.
Parker, Jonathan Earl.
Bedvic, Thomas S.
Application Number:
JP50441296A
Publication Date:
June 30, 1998
Filing Date:
July 07, 1995
Export Citation:
Assignee:
La Jolla Cancer Research Foundation
International Classes:
A61K38/00; A61P35/00; A61P43/00; B01D41/04; C07K14/00; C07K14/47; C07K14/78; C12N15/09; C12Q1/00; G01N30/06; G01N30/26; G01N30/46; G01N30/88; G01N30/02; G01N30/96; G01N33/53; G01N33/68; (IPC1-7): C12N15/09; B01D41/04; C07K14/00; C07K14/78; C12Q1/00; G01N30/02; G01N30/06; G01N30/26; G01N30/46; G01N30/88; G01N30/96
Attorney, Agent or Firm:
Shusaku Yamamoto