Title:
【発明の名称】微細加工した検出構造体
Document Type and Number:
Japanese Patent JP3298882
Kind Code:
B2
Abstract:
Disclosed are devices and methods for the clinical analysis of a sperm sample. The devices comprise a solid substrate, typically on the order of a few millimeters thick and approximately 0.2 to 2.0 centimeters square, microfabricated to define a sample inlet port (16A) and a mesoscale flow channel (20, 40) extending from the inlet port (16A). In one embodiment, a sperm sample is applied to the inlet port (16A), and the competitive migration of the sperm sample through the mesoscale flow channel (20, 40) is detected to serve as an indicator of sperm motility. In another embodiment, the substrate of the device is microfabricated with a sperm inlet port (16A), an egg nesting chamber (16B), and an elongate mesoscale flow channel (20, 40) communicating between the egg nesting chamber (16B) and the inlet port (16A). In this embodiment, a sperm sample is applied to the inlet port, and the sperm in the sample is permitted to competitively migrate from the inlet port (16A) through the channel (20, 40) to the egg nesting chamber (16B), where in vitro fertilization occurs. The devices of the invention may be used in a wide range of applications in the analysis of a sperm sample, including the analysis of sperm morphology or motility, to assess sperm binding properties, and for in vitro fertilization.
Inventors:
Wilding, Peter
Clicka, Larry Jay
Zemel, JN
Clicka, Larry Jay
Zemel, JN
Application Number:
JP51949993A
Publication Date:
July 08, 2002
Filing Date:
April 29, 1993
Export Citation:
Assignee:
Trustees of the University of Pennsylvania
International Classes:
B01D61/18; B01D67/00; B01D71/02; B01J19/00; A01K67/02; B01L3/00; B01L7/00; C12M1/00; C12M1/26; C12M1/34; C12M3/00; C12M3/08; C12N15/09; C12Q1/68; G01N11/04; G01N27/07; G01N33/483; G01N33/50; G01N33/543; G01N37/00; A61B17/04; B01F5/06; B01L9/00; C40B40/06; (IPC1-7): G01N33/483; B01L3/00; C12M1/34; C12M3/08; C12Q1/68; G01N33/543
Domestic Patent References:
| JP62129759A | ||||
| JP3223674A | ||||
| JP367171A | ||||
| JP6370165A |
Other References:
【文献】国際公開90/9596(WO,A1)
Attorney, Agent or Firm:
Aoyama Ryo (1 person outside)