Title:
【発明の名称】新規なNAD合成酵素およびその製造法
Document Type and Number:
Japanese Patent JP2579319
Kind Code:
B2
Abstract:
A method of assaying a specimen for ATP, deamide-NAD, ammonia including ammonia in the form of an ammonium ion or a component of a reaction system in which ATP, deamide-NAD or ammonia including ammonia in the form of an ammonium ion is generated or consumed, which method comprises: (i) incubating the specimen with a NAD synthetase having the following properties: (1) Action: at least catalyses in the presence of Mg<+><+> on Mn<+><+> ion the reaction (a): and does not catalyse in the presence of Mg<+><+> ion the reaction (b): (2) Substrate specificity: utilises ammonia, including ammonia in the form of an ammonium ion, as a substrate but does not utilise glutamine or asparagine as a substrate; in the present of Mg<+><+> or Mn<+><+> ions and the two of ATP, deamide-NAD and ammonia which may be in the form of an ammonium ion which are not being assayed for or which are not generated or consumed by the said reaction system, to generate thereby NAD; (ii) subjecting the thus-generated NAD to a coenzyme cycling reaction comprising reducing said generated NAD to reduced NAD and oxidizing the thus-generated reduced NAD to NAD; and (iii) measuring a compound which is consumed or generated in said cycling reaction.
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Inventors:
TAKAHASHI MAMORU
MISAKI HIDEO
IMAMURA SHIGEYUKI
MATSURA KAZUO
MISAKI HIDEO
IMAMURA SHIGEYUKI
MATSURA KAZUO
Application Number:
JP19101887A
Publication Date:
February 05, 1997
Filing Date:
July 30, 1987
Export Citation:
Assignee:
ASAHI CHEMICAL IND
International Classes:
C12N9/00; C12P1/00; C12P3/00; C12Q1/00; C12Q1/48; C12R1/07; (IPC1-7): C12N9/00