Title:
【発明の名称】オリゴ糖-タンパク質複合体及びその製造方法
Document Type and Number:
Japanese Patent JPH05507487
Kind Code:
A
Abstract:
This invention relates to a method of producing peracetyloxazolines from peracetyl saccharides. The method involves reacting the starting material, a peracetyl saccharide, with a reagent combination, to directly produce the peracetyl oxazoline. This method may be used for the activation of oligosaccharides, wherein an oligosaccharide containing a reducing GlcNAc terminus is activated by the formation of an oxazoline at the terminal GlcNAc, and then coupled with a bifunctional spacer to provide an oligosaccharide-spacer conjugate. The activated oligosaccharide-spacer conjugate is then coupled to a protein, such as granulocyte colony stimulating factor or gamma -interferon, providing a neoglycoprotein conjugate. The invention provides a method for forming neoglycoprotein conjugates which may improve biological and physiochemical properties of the protein. For example, serum life-time or efficiency of drug delivery of the peptide to a target organ or cell may be improved.
Inventors:
Colon, Marcelo
Davis, Jeffrey Tea
Rasmussen, James Earl
Borrowski, Marian
One, Barbara Wye
Hirani, Sirish
Davis, Jeffrey Tea
Rasmussen, James Earl
Borrowski, Marian
One, Barbara Wye
Hirani, Sirish
Application Number:
JP51064591A
Publication Date:
October 28, 1993
Filing Date:
May 24, 1991
Export Citation:
Assignee:
GENZYME CORPORATION
International Classes:
A61K47/48; C07H9/06; C07H13/04; C07H15/04; C07H15/12; C07K1/113; C07K1/22; C07K14/00; C07K14/52; C07K14/53; C07K14/535; C07K14/555; C07K14/57; C07K14/76; (IPC1-7): C07H9/06; A61K47/48; C07H15/04; C07K15/14
Attorney, Agent or Firm:
Yuzo Yamazaki (2 outside)