PURPOSE: To obtain the DNA fragment containing a gene coding dihydrodipicolinic acid reductase from Brevibacterium-flavum, and capable of transforming a Coryne type bacteria to produce useful products such as L-lysine, etc., in a high efficiency.
CONSTITUTION: A noval DNA fragment expressed by formula, etc., containing a gene coding dihydrodipicolinic acid reductase (EC-1. 3. 1. 26), derived from a Brevibacterium-flavum [[e.g. Brevibacterium-flavum MJ-233 (FERM-BP-1497), etc.]. The enzyme is obtained as a form of an objective DNA fragment, by culturing Brevibacterium-flavum MJ-233 until the logarithmic growth phase, collecting the microbial cells, suspending them in a solution containing a lysozyme, further adding protease K and a surface active agent to subject them to bacteriolysis, collecting genes from the bacteriolytic solution by a conventional procedure, treating the genes with a restriction enzyme, and selecting a DNA coding dihydrodipicolinic acid reductase.
KOBAYASHI MIKI
YUGAWA HIDEAKI
