Title:
IMMOBILIZATION OF GLUCOSE ISOMERASE
Document Type and Number:
Japanese Patent JPS5840087
Kind Code:
A
Abstract:
PURPOSE: Cobalt ion and magnesium ion are added to the coagulation bath to give an immobilized glucose isomerase with stabilized activity for a long period of time.
CONSTITUTION: A water-soluble cobalt compound and a magnesium compound are added to a glucose isomerase aqueous solution of an arbitrary concentration so that the concentration of the cobalt ion becomes 1W70 preferably 5W50, micrograms per 1mg of the protein of glucose isomerase enzyme and magnesium ion, 3W350, preferably 10W100 micrograms. Then, a fibrous or powdery support, preferably having amino groups, is dipped into the resultant immobilization bath and stood one overnight at about 30°C.
Inventors:
ICHIJIYOU HISAO
SUEHIRO TETSUROU
YAMAUCHI AIZOU
OGAWA SHIGEO
SHIRASAKA AKIHISA
YAMADA SETSUO
SUEHIRO TETSUROU
YAMAUCHI AIZOU
OGAWA SHIGEO
SHIRASAKA AKIHISA
YAMADA SETSUO
Application Number:
JP13910381A
Publication Date:
March 08, 1983
Filing Date:
September 03, 1981
Export Citation:
Assignee:
KOGYO GIJUTSUIN
NICHIBI KK
NICHIBI KK
International Classes:
C12N9/92; C12N11/08; (IPC1-7): C12N9/92; C12N11/08
Attorney, Agent or Firm:
Agata Akira
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