Title:
【発明の名称】ヘルペスウイルスチミジンキナーゼをコード付けするDNAの単離方法
Document Type and Number:
Japanese Patent JPH04500007
Kind Code:
A
Abstract:
Methods for isolating thymidine kinase-encoding DNA of a herpes virus are described. These methods utilize degenerate primers based on regions of relatively conserved amino acid sequence in herpes virus thymidine kinase proteins to initiate a polymerase chain reaction which yields large amounts of the thymidine kinase-encoding DNA. The methods are illustrated in the isolation of the thymidine kinase gene of feline herpes virus, which can be used to construct recombinant thymidine kinase-negative feline herpes viruses for purposes of constructing live vaccines and expression vectors. In addition, the regulatory elements of the feline herpes virus thymidine kinase gene are useful in the construction of recombinant DNA vectors.
Inventors:
Post, Leonard Y.
Compton, Teresa
Nurnberg, Jack H
Petrovskies, Eric A.
Compton, Teresa
Nurnberg, Jack H
Petrovskies, Eric A.
Application Number:
JP50923289A
Publication Date:
January 09, 1992
Filing Date:
August 02, 1989
Export Citation:
Assignee:
PHARMACIA&UPJOHN COMPANY
Citas Corporation
Citas Corporation
International Classes:
A61K39/245; A61P31/22; C07K14/03; C07K14/15; C12N15/09; C12N7/00; C12N9/12; C12N15/00; C12N15/10; C12N15/869; A61K38/00; C12R1/92; (IPC1-7): A61K39/245; C12N7/01; C12N15/10; C12N15/54; C12N15/86
Attorney, Agent or Firm:
Aoyama Ryo (1 person outside)