To provide a method of manufacturing a section observation sample, where damage and physical modification when manufacturing a section observation sample by a freezing fracture method have been reduced, and to provide a fixing tool for manufacturing even a minute section or a very small amount of section easily and simply.

The fixing tool for manufacturing a section observation sample by breaking a film-like or sheet-like sample using the freezing fracture method includes a base material brought into contact with one surface of the film-like or sheet-like sample and a fixing tool for fixing both the ends of the sample to the base material, where the base material is bent.