To provide a method for transformation of yeast that can simply treat a large number of strains in no need of preparation of competent cells.
In the method for producing a transformant by transforming fission yeast with a DNA for transformation, the yeast cell bodies cultured on a solid medium is added to the treating solution including a DNA for the transformation, a carrier DNA, polyethylene glycol and a buffer solution including a lithium salt in an amount of more than 1 x 107 per 1 μg of DNA for transformation. Then, the mixture is incubated for more than 30 minutes and the treated solution contains 0.5 to 10 μg of the DNA for transformation per 100 μL of the treated solution and the carrier DNA more than 20 fold mass based on the DNA for transformation.
HAMA YUKO
MORITA TOMOTAKE
