Title:
トランスポゾン系、それを含むキット及びそれらの使用
Document Type and Number:
Japanese Patent JP6956416
Kind Code:
B2
Abstract:
An in vitro method for integrating an exogenous DNA sequence into the genome of a cell using a transposon system. The transposon system includes a first vector carrying inverted repeat sequences and an exogenous DNA, and a second vector that expresses a transposases. Also provided is a kit including the transposon system for integrating an exogenous DNA into the genome of a cell. A method for treating a subject is described that includes engineering an immune cell to carry an exogenous DNA sequence using the method and/or the kit described above and administering an effective amount of the engineered immune cell to the subject.
Inventors:
Woo Saleina Chun-Yuan
Application Number:
JP2018524760A
Publication Date:
November 02, 2021
Filing Date:
December 12, 2016
Export Citation:
Assignee:
Genome Frontier Therapeutics, Incorporated
International Classes:
C12N15/90; A61K35/17; A61K48/00; A61P43/00; C12N15/85
Domestic Patent References:
| JP2008545375A |
Foreign References:
| CN102943092A | ||||
| WO2015157386A1 | ||||
| US20090042297 |
Other References:
Meir YJ. et al.,Genome-wide target profiling of piggyBac and Tol2 in HEK 293: pros and cons for gene discovery and gene therapy,BMC Biotechnology,2011年,11,28
Meir YJ. et al.,A versatile, highly efficient, and potentially safer piggyBac transposon system for mammalian genome manipulations,The FASEB Journal,2013年,27(11),4429-4443
Nat Biotechnol. 2010 Dec (Epub 2010 Nov 21), vol. 28, no. 12, pp. 1287-1289 (online methods pp. 1-2)
Mol Ther. 2003 Sep, vol. 8, no. 3, pp. 495-500
Meir YJ. et al.,A versatile, highly efficient, and potentially safer piggyBac transposon system for mammalian genome manipulations,The FASEB Journal,2013年,27(11),4429-4443
Nat Biotechnol. 2010 Dec (Epub 2010 Nov 21), vol. 28, no. 12, pp. 1287-1289 (online methods pp. 1-2)
Mol Ther. 2003 Sep, vol. 8, no. 3, pp. 495-500
Attorney, Agent or Firm:
Patent Business Corporation Saegusa International Patent Office
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