Title:
【発明の名称】組換えDNA分子
Document Type and Number:
Japanese Patent JP2540031
Kind Code:
B2
Abstract:
A description is given of a cloning vector useful for the expression and secretion of heterologous proteins in Bacillus subtilis, molecules of recombinant DNA formed by the said cloning vector, expediently cut at a restriction site located within the neutral protease gene, and by the sequence of heterologous DNA which codes for the protein of interest, Bacillus subtilis cells transformed with the said molecules and capable of expressing the sequence of heterologous DNA and of producing and secreting the protein coded by the said sequence of heterologous DNA in high yields.The proteins obtained in this manner are useful in the fields of pharmaceuticals and foods, or as compounds for the production of substances which are normally obtained by chemical synthesis.
Inventors:
SARUBATOORE TOOMA
MARIINA DERUBUUE
GUIIDO GURANDEI
ANTONIO MEERE
MARIINA DERUBUUE
GUIIDO GURANDEI
ANTONIO MEERE
Application Number:
JP16099886A
Publication Date:
October 02, 1996
Filing Date:
July 10, 1986
Export Citation:
Assignee:
ENIRICERCHE SPA
International Classes:
C07K14/585; C12N1/20; C12N1/21; C12N9/54; C12N15/00; C12N15/75; C12P21/02; C12N15/09; C12R1/125; (IPC1-7): C12N15/09; C12N1/21; C12P21/02
Domestic Patent References:
| JP58500590A | ||||
| JP6156080A | ||||
| JP61181374A |
Attorney, Agent or Firm:
Kimura Masami