Title:
細胞融合用の不平等電界チャンバ
Document Type and Number:
Japanese Patent JP4768441
Kind Code:
B2
Abstract:
An apparatus is provided for carrying out fusion of biological cells (10) and includes a base member (24) on which a conductive outer electrode (18) is supported and has an outer electrode radius (r2) and has an electrode height (19). A conductive inner electrode (20) is supported on the base member (24) and has an inner electrode radius (r1) and also has the electrode height (19). The outer and inner electrodes (18, 20) are spaced apart from each other by a gap which defines a fusion chamber (14). The inner electrode radius (r1), the outer electrode radius (r2), and the gap are selected in accordance with a predetermined range of selectable ratios (rl/r2) in a range from 0.7 to 0.9, wherein a selected gap is limited by the range of selectable ratios (rl/r2), and wherein a determined ratio (rl/r2) among the selectable ratios is based on the selected gap, such that compression between the biological cells (10) and permeability between cell membranes are maximized and temperature rise is minimized for providing cell fusion in the fusion chamber (14).
Inventors:
Walters, Richard, Yee.
Application Number:
JP2005513115A
Publication Date:
September 07, 2011
Filing Date:
December 01, 2003
Export Citation:
Assignee:
Selectis Societe Anonymous
International Classes:
C12M1/42; C12M1/00; C12M3/00; C12N5/07; C12N5/071; C12N5/09; C12N13/00; C12N1/00
Domestic Patent References:
| JPS62172400U | 1987-10-31 | |||
| JPS62257379A | 1987-11-09 |
Foreign References:
| US20030082163A1 | 2003-05-01 |
Attorney, Agent or Firm:
Atsushi Aoki
Takashi Ishida
Fukumoto product
Tetsuji Koga
Yoichi Watanabe
Masaru Nakajima
Ryotaro Takei
Takashi Ishida
Fukumoto product
Tetsuji Koga
Yoichi Watanabe
Masaru Nakajima
Ryotaro Takei