Title:
USE OF ENZYME
Document Type and Number:
Japanese Patent JPS59210891
Kind Code:
A
Abstract:
The invention relates to an enzymatic process in which an immobilised biocatalyst containing an enzyme catalysing the formation of a proton generating product, and containing an enzyme catalysing the formation of a proton consuming product, is contacted with a medium containing substrates for the enzymes present in the immobilised biocatalyst. One of the enzymatic reactions is controlled in such a way that favourable pH conditions for the other reaction are generated in the biocatalyst. For example, the urease catalysed hydrolysis of urea can be used to generate nearly optimum pH conditions for the penicillin acylase catalysed deacylation of penicillins. This internal pH control is superior to controlling the pH of the substrate medium by addition of buffers.
Inventors:
ITSUHAKU RUSOO
Application Number:
JP7762384A
Publication Date:
November 29, 1984
Filing Date:
April 19, 1984
Export Citation:
Assignee:
TNO
International Classes:
C12N11/18; C12P1/00; C12P37/06; C12N11/04; (IPC1-7): C12N11/04; C12N11/18; C12P1/00
Attorney, Agent or Firm:
Heiyoshi Odashima
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