Title:
Method for imparting acid and salt resistance and production of useful substances using acid and salt resistant yeast
Document Type and Number:
Japanese Patent JP6343754
Kind Code:
B2
Abstract:
Provided are: a method for preparing yeast presenting various types of growth inhibition resistance and fermentation inhibition resistance in strong-acid (low-pH) and high-salt-concentration environments by introducing genes and proteins presenting resistance to acid and salt derived from acid-resistant, salt-resistant yeast into yeast having exceptional fermentation production or by improving the expression level of such attributes, whereby acid resistance and salt resistance are imparted; and a method for producing useful substances such as ethanol by using this method. Genetically modified yeast constantly expressing an LGS1 gene derived from Issatchenkia orientalis or an LGS1 homologous gene (GAS1, PHR1, or PHR2 gene, or the like) belonging to the GH72 family and derived from another yeast, a method for achieving resistance in low-pH and high-salt-concentration environments using the same, and a method for highly efficiently producing useful substances such as ethanol and the like from glucose or a saccharified solution containing glucose.
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Inventors:
Matsunori Akinori
Tetsuya Goto
Tamotsu Hoshino
Tetsuya Goto
Tamotsu Hoshino
Application Number:
JP2016508752A
Publication Date:
June 20, 2018
Filing Date:
March 18, 2015
Export Citation:
Assignee:
National Institute of Advanced Industrial Science and Technology
International Classes:
C12N1/19; C12P7/06
Domestic Patent References:
| JP2008043234A | ||||
| JP2012510808A | ||||
| JP2009195220A |
Other References:
CHAN, Giek Far et al.,Genome Sequence of Pichia kudriavzevii M12, a Potential Producer of Bioethanol and Phytase,Eukaryot. Cell,2012年,Vol. 11,pp. 1300-1301
Database GenBank [online], Accession No. BAO37942, 15-Jan-2014 uploaded, [retrieved on 2015-05-18], DEFINITION: probable 1, 3-beta-glucanosyltransferase GAS3 [Kluyveromyces marxianus DMKU3-1042],URL,http://www.ncbi.nlm.nih.gov/protein/574140147?sat=3&satkey=23825876
JEFFRIES, Thomas W et al.,Genome sequence of the lignocellulose-bioconverting and xylose-fermenting yeast Pichia stipitis,Nat. Biotechnol.,2007年,Vol. 25,pp. 319-326
Database GenBank [online], Accession No. BAO37942, 15-Jan-2014 uploaded, [retrieved on 2015-05-18], DEFINITION: probable 1, 3-beta-glucanosyltransferase GAS3 [Kluyveromyces marxianus DMKU3-1042],URL,http://www.ncbi.nlm.nih.gov/protein/574140147?sat=3&satkey=23825876
JEFFRIES, Thomas W et al.,Genome sequence of the lignocellulose-bioconverting and xylose-fermenting yeast Pichia stipitis,Nat. Biotechnol.,2007年,Vol. 25,pp. 319-326
Attorney, Agent or Firm:
Yusuke Hiraki
Satoshi Fujita
Natsuo Tanaka
Takashima Ejima
Satoshi Fujita
Natsuo Tanaka
Takashima Ejima