VEGETABLE PROTEIN EXTRACT AND ISOLATED SUBSTANCE RICH IN AGLUCONE ISOFLAVONE AND THEIR PRODUCTION

Japanese Patent JP3609273

B2

PROBLEM TO BE SOLVED: To produce the subject isolated substance capable of recovering isoflavone in a high ratio because the isoflavone is contained in the form of an aglucone having a low solubility by abundantly including genistein which is an aglucone isoflavone without glucone bonded thereto.
SOLUTION: This isolated substance has at least 1.5 mg/g genistein content based on the dry weight, preferably about 1.5 to about 3.5 mg/g genistein content and about 1.0 to about 3.0 mg/g daidzein content respectively based on the dry weight. The objective isolated substance is preferably obtained by subjecting a vegetable proteinaceous material containing a glucone isoflavone to an extraction treatment with an aqueous extracting agent having a higher pH than the isoelectric point of the material, then reacting a β-glucosidase enzyme with the resultant extract and converting the glucone isoflavone into the aglucone isoflavone. A soybean flake freed of an oil by solvent extraction is preferred as the material. In this case, the pH of the aqueous extracting agent is preferably 6.7-9.7.

Jerome El Shen
Barbara Abryan

JP31173798A

January 12, 2005

November 02, 1998

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PROTEIN TECHNOLGIES INTERNATIONAL INCORPORATED

A61K38/00; A23J1/14; A23J3/14; A23J3/16; A23J3/34; A23L1/305; A61K31/00; A61K31/35; A61K36/48; A61K38/01; A61P35/00; C07D311/36; C07D311/40; C07K14/415; C12P17/06; (IPC1-7): C07K14/415; A23J3/16; A23L1/305

JP9023822A
JP1258669A

WO1997037547A1

Minoru Nakamura
Fumiaki Otsuka
Shishido Kaichi
Hideto Takeuchi
Toshio Imajo
Nobuo Ogawa
Village shrine Atsuo

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