Title:
Vitrification preservation solution of cells
Document Type and Number:
Japanese Patent JP6333513
Kind Code:
B2
Abstract:
The vitrification medium for cells according to the present invention is a vitrification medium for cells comprising a cell membrane permeable substance and a cell membrane non-permeable substance, in which, the content of the cell membrane permeable substance is in the range of 30 to 50% by volume, and the osmotic pressure generated in association with the cell membrane non-permeable substance, which is a fraction of the total osmotic pressure on the cell membrane on suspending the cells in the vitrification medium, is in the range of 280 mOsm or more. The vitrification medium for cells according to the present invention is excellent in operability and safety.
Inventors:
Kunitomi Yoshihiro
Masahiro Sasaki
Masahiro Sasaki
Application Number:
JP2012544326A
Publication Date:
May 30, 2018
Filing Date:
November 18, 2011
Export Citation:
Assignee:
SEIREN CO.,LTD.
International Classes:
A01N1/02; C12N5/071; C12N5/0735
Domestic Patent References:
| JP2002525064A | ||||
| JP5503706A | ||||
| JP2007217385A | ||||
| JP2006115837A |
Foreign References:
| WO2005045007A1 |
Other References:
Biology of Reproduction, 2010年 (published on line before print 14 October 2009),Vol.82,p.444-450
J. Reproduction and fertility, 1994年,Vol.100,p.123-129
Cryobiology, 1999年,Vol.38,p.119-130
J. Reproduction and fertility, 1994年,Vol.100,p.123-129
Cryobiology, 1999年,Vol.38,p.119-130
Attorney, Agent or Firm:
Konno Akio
Minoru Inami
Takeyasu Ito
Keisei Tamura
Minoru Inami
Takeyasu Ito
Keisei Tamura