細胞シグナル伝達経路のサイトメトリー解析のための全血製剤

Japanese Patent JP5058802

B2

This invention is directed to a method for preparation of a biological sample for measurement of protein epitopes that allows for the preservation of intracellular protein epitopes and detection of signal transduction pathways based on the ability to capture transient activation states of the epitopes. The method provided by the invention allows for the rapid fixation of biological samples containing red blood cells, to ensure that epitopes of signal transduction molecules and other intracellular protein epitopes are preserved in the active state. The method of the invention further allows for lysis of red blood cells, thereby making it a useful method for cytometric analysis of biological samples, including, for example, whole blood, bone marrow aspirates, peritoneal fluids, and other red blood cell containing samples. The invention also provides a method to recover or “unmask” epitopes on intracellular antigens that have been made inaccessible by the cross linking fixative necessary to fix the sample. Significantly, the methods of the invention allow preservation and analysis of phospho-epitope levels in biological samples taken directly from patients to determine disease-specific characteristics.

Butterfly, sue
Hedley, David
Shanky, Tea. Vincent
Grom, Patricia

JP2007529916A

October 24, 2012

August 12, 2005

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BECKMAN COULTER,INCORPORATED
GENZYME CORPORATION

G01N33/48; C12Q1/04; G01N15/00; G01N21/64; G01N21/78; G01N33/49; G01N33/53; G01N33/543

JP6078792A
JP62071857A
JP8308593A
JP2000039433A
JP2002534731A

US20050084924
US20040048384
US20050042688

Hidesaku Yamamoto
Takaaki Yasumura
Natsuki Morishita

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