【発明の名称】ハイドロキシアパタイトへの吸着クロマトグラフィーによる初乳の処理方法、得られた初乳の活性フラクション、および活性フラクションを含む細胞培地

Title:

Document Type and Number:

Japanese Patent JPH087201

Kind Code:

B2

Abstract:

According to the method, the preferably skimmed colostrum is contacted with a cation exchanger such as a sulphonic-function resin, the portion separated from the exchanger by elution is subjected to hydroxyapatite adsorption, and the fraction retained on the hydroxyapatite is recovered by elution, by washing with a phosphate pad at a maximum concentration of 500 mM, preferably about 100 mM. The retained, active fraction contains more than half of the growth factors of the initial colostrum, but almost no proteases, and can be used as an additive for cell culture media, particularly as a substitute for foetal calf serum.

Inventors:

Kanke Bernard
Les Hérenes
Spic Genevieve
Montreal Jean
Toma daniel

Application Number:

JP51111391A

Publication Date:

January 29, 1996

Filing Date:

June 24, 1991

Export Citation:

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Assignee:

Clar (Essa Ale El)

International Classes:

B01D15/04; A23C7/00; A23C9/146; A23C9/20; B01D15/08; C07K14/475; C12N5/00; C12N5/02; C12N5/07; C12N5/071; C12N5/10; G01N30/02; G01N30/88; (IPC1-7): G01N30/88; A23C7/00; B01D15/08; C12N5/06; G01N30/02

Attorney, Agent or Firm:

Norio Ogo

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