Title:
The cell culture platform for unicellular sorting, and reinforcement of re-programming of iPSC
Document Type and Number:
Japanese Patent JP6182456
Kind Code:
B2
Abstract:
The invention provides cell culture conditions for culturing stem cells, including feeder-free conditions for generating and culturing human induced pluripotent stem cells (iPSCs). More particularly, the invention provides a culture platform that allows long-term culture of pluripotent cells in a feeder-free environment; reprogramming of cells in a feeder-free environment; single-cell dissociation of pluripotent cells; cell sorting of pluripotent cells; maintenance of an undifferentiated status; improved efficiency of reprogramming; and generation of a naïve pluripotent cell.
Inventors:
Valamar, Bharam
Abjarur, Ramsey
Flynn, Peter
Abjarur, Ramsey
Flynn, Peter
Application Number:
JP2013546290A
Publication Date:
August 23, 2017
Filing Date:
December 19, 2011
Export Citation:
Assignee:
Fate Therapeutics, Incorporated
International Classes:
C12N5/10; C12N5/07; C12N5/071; C12N15/09; C12Q1/04
Domestic Patent References:
| JP2010504090A |
Foreign References:
| WO2010077955A1 | ||||
| WO2010120785A1 | ||||
| WO2011056971A1 |
Other References:
ZHU, S., et al.,Cell Stem Cell,2010年12月 3日,7,pp.651-655,URL,http://dx.doi.org/10.1016/j.stem.2010.11.015
長田翔伍ら,Generation of Ground State Human Induced Pluripotent Stem Cells with Kinase Inhibitors,第83回日本生化学会大会、第33回日本分子生物学会年会合同大会講演要旨集(CD-ROM),2010年11月19日,要旨番号4P-0877
LIN, T., et al.,Nature Methods,2009年10月18日,6(11),pp.805-808
長田翔伍ら,Generation of Ground State Human Induced Pluripotent Stem Cells with Kinase Inhibitors,第83回日本生化学会大会、第33回日本分子生物学会年会合同大会講演要旨集(CD-ROM),2010年11月19日,要旨番号4P-0877
LIN, T., et al.,Nature Methods,2009年10月18日,6(11),pp.805-808
Attorney, Agent or Firm:
Yoshiyuki Inaba
Toshifumi Onuki
Akihiko Eguchi
Kazuhiko Naito
Toshifumi Onuki
Akihiko Eguchi
Kazuhiko Naito