COMPOSITIONS, METHODS AND KITS FOR AMPLIFYING NUCLEIC ACID

Title:

COMPOSITIONS, METHODS AND KITS FOR AMPLIFYING NUCLEIC ACID

Document Type and Number:

Japanese Patent JP2016192979

Kind Code:

A

Abstract:

PROBLEM TO BE SOLVED: To provide compositions, methods and kits for amplifying nucleic acid.SOLUTION: The disclosure is directed to compositions, methods and kits for amplifying a target nucleic acid while reducing non-specific fluorescence and undesirable amplification products (sometimes referred to as secondary amplification products or spurious side-products). The enzyme inhibitor disclosed herein comprises a nucleotide sequence and at least one quencher. Also provided is a complex comprising an enzyme inhibitor associated with the enzyme, wherein at least one enzymatic activity of the enzyme is inhibited. Methods for amplifying a target nucleic acid while reducing undesired amplification products, as well as methods for reducing non-specific fluorescence are disclosed. Kits for expediting the performance of certain disclosed methods are also provided.SELECTED DRAWING: None

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Inventors:

DONG SHOULIAN
STEVENS JUNKO
LEE DANNY H

Application Number:

JP2016162389A

Publication Date:

November 17, 2016

Filing Date:

August 23, 2016

Export Citation:

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Assignee:

APPLIED BIOSYSTEMS LLC

International Classes:

C12N15/115; C12N15/09; C12Q1/68

Attorney, Agent or Firm:

Hidesaku Yamamoto
Natsuki Morishita

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