Title:
Pluripotent stem cell culture system and pluripotent stem cell passage method
Document Type and Number:
Japanese Patent JP6292415
Kind Code:
B2
Abstract:
[Object] To provide a system that is suitable for culturing a large amount of pluripotent stem cells. [Solution] A culture system (10) provided with: a culture bag (11) for suspending and culturing pluripotent stem cells in a culture medium; a waste liquid container (12) for storing used culture medium that is connected to the culture bag (11); a fresh medium container (13) for storing a fresh culture medium that is connected to the culture bag (11); three-way stopcocks (51 to 55) for switching flow channels from the culture bag (11) to the waste liquid container (12) or the fresh culture medium container (13), etc.; a trap portion (14) for trapping the pluripotent stem cells in the culture medium in the flow toward the waste liquid container (12) side; a filter portion (15) for subculturing that is formed in fourth and fifth flow channels (45) in parallel to a first flow channel (41), the first flow channel (14) connecting the trap part (14) to the culture bag (11), and provided with a mesh (31) by which a cell mass of the pluripotent stem cells can be divided; and a pump (16) for flowing the liquid in the individual flow channels.
Inventors:
Norio Nakatsuji
Yoshihiro Yoshikawa
Seisuke Takeuchi
Tachiyama Daiki
Yoshihiro Yoshikawa
Seisuke Takeuchi
Tachiyama Daiki
Application Number:
JP2015504235A
Publication Date:
March 14, 2018
Filing Date:
February 20, 2014
Export Citation:
Assignee:
Kyoto University
NIPRO CORPORATION
NIPRO CORPORATION
International Classes:
C12M3/02; C12M1/00; C12M3/06; C12N5/0735; C12N5/10
Domestic Patent References:
| JP2010526530A | ||||
| JP2008079554A | ||||
| JP2005198626A | ||||
| JP1005486A | ||||
| JP6007500U |
Foreign References:
| WO2012002497A1 | ||||
| WO2011058558A2 | ||||
| EP1903101A1 |
Attorney, Agent or Firm:
Nobuo Nishiki
Tomohiro Matsuda
Tomohiro Matsuda