Title:
Test substance detection method and reagent kit for test substance detection
Document Type and Number:
Japanese Patent JP6320651
Kind Code:
B2
Abstract:
Provided is a method for detecting a test substance, the method including the steps of: forming an immune complex on a first solid phase by bringing a test substance, a labeled antibody, a capture antibody and the first solid phase into contact with one another; transferring the immune complex onto a second solid phase by breaking the binding between the capture antibody and the first solid phase to liberate the immune complex, and bringing the second solid phase and the immune complex into contact with each other, where the second solid phase is bound to the capture antibody; and detecting the test substance by measuring a label contained in the complex on the second solid phase. The test substance detected by the method is a multimeric antigen, particularly amyloid ² or tau protein.
Inventors:
Toshihiro Watanabe
Application Number:
JP2017554615A
Publication Date:
May 09, 2018
Filing Date:
February 06, 2017
Export Citation:
Assignee:
Sysmex Corporation
International Classes:
G01N33/543
Domestic Patent References:
| JP2008530578A | ||||
| JP200985685A | ||||
| JP2001116571A |
Foreign References:
| WO2009011500A2 |
Other References:
LEVINE H III,Alzheimer's β-peptide oligomer formation at physiologic concentrations,Anal Biochem,2004年12月 1日,Vol.335 No.1,Page.81-90
Attorney, Agent or Firm:
Shintaro Nogawa
Shinji Kai
Yusuke Kaneko
Kiyoshi Inamoto
Shinji Kai
Yusuke Kaneko
Kiyoshi Inamoto