Title:
流体状態における硬化剤の酵素的生産方法
Document Type and Number:
Japanese Patent JP4295610
Kind Code:
B2
Abstract:
The invention relates to a method for the enzymatic production of a curing agent in its fluid state, e.g. liquid, comprising, in free phase, at least one oxygenated chemical species. Said method consists in bringing into contact at least one enzymatic catalysts agent, comprising at lease one peroxidase-type enzyme; an oxidizable substrate in aqueous phase that can be oxidized by the action of an oxygen donor, by catalysis by said enzymatic catalysis agent, generating said oxygenated chemical species in free phase; and said oxygen donor. The inventive method is characterized in that: e) an aqueous reaction bath is formed comprising, in addition to the oxidizable substrate and the oxygen donor, said enzymatic catalysis agent in divided solid phase, but in free phase, distributed is said bath, which may be set in motion; f) the aqueous reaction bath is separated into a fraction enriched with the enzymatic catalysis agent in divided solid phase and a fraction free from said catalysis agent, from which the curing agent is obtained.
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Inventors:
Herve Case
Application Number:
JP2003500244A
Publication Date:
July 15, 2009
Filing Date:
May 31, 2002
Export Citation:
Assignee:
Temuy Europe
International Classes:
C12N9/08; A61L2/16; C12M1/40; C12N9/02; C12N11/00; C12P3/00
Domestic Patent References:
| JP58152486A | ||||
| JP64061427A | ||||
| JP11506444A | ||||
| JP63098382A | ||||
| JP49025128A |
Foreign References:
| US4978528 |
Other References:
千畑一郎編,固定化酵素,株式会社講談社,1975年11月 1日,第1版第2刷,p.1-8
Attorney, Agent or Firm:
Masatake Shiga
Takashi Watanabe
Yasuhiko Murayama
Shinya Mitsuhiro
Takashi Watanabe
Yasuhiko Murayama
Shinya Mitsuhiro