Title:
減圧処理/加圧処理の使用を含むエレクトロポーレーション方法
Document Type and Number:
Japanese Patent JP4273231
Kind Code:
B2
Abstract:
An object of the present invention is to establish a transformation method simpler and more rapid than conventional techniques. This object can be solved by a method comprising the steps of a) holding a cell under a pressure different from an atmospheric pressure, and b) placing the cell and a nucleic acid under conditions capable of inducing electroporation. The present invention removes the necessity of culture which is conventionally required after gene transfer. Therefore, the present invention makes it possible to obtain a transformant of species which are impossible or considerably difficult to transform by conventional techniques. The simple transformation method of the present invention easily allows large-scale processing and large-scale analysis for research and development in the art. In addition, the present invention triggers dramatic advances in research, potentially leading to the development of innovative recombinants.
Inventors:
Takashi Hagio
Yutaka Tabei
Yutaka Tabei
Application Number:
JP2004521208A
Publication Date:
June 03, 2009
Filing Date:
July 14, 2003
Export Citation:
Assignee:
NATIONAL INSTITUTE OF AGROBIOLOGICAL SCIENCES
International Classes:
C12N15/09; C12M1/00; C12M1/42; C12N15/82; C12N15/87
Foreign References:
| WO2000063408A1 | ||||
| WO2001005994A1 |
Attorney, Agent or Firm:
Hidesaku Yamamoto
Takaaki Yasumura
Natsuki Morishita
Takaaki Yasumura
Natsuki Morishita
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