COMPLETE GENE (CEFG) ENCODING ACETYL-COENZYME A: DEACETYL CEPHALOSPORIN C ACETYLTRANSFERASE OF CEPHALOSPORIUM ACREMONIUM, ITS ISOLATION AND USE

Title:

COMPLETE GENE (CEFG) ENCODING ACETYL-COENZYME A: DEACETYL CEPHALOSPORIN C ACETYLTRANSFERASE OF CEPHALOSPORIUM ACREMONIUM, ITS ISOLATION AND USE

Document Type and Number:

Japanese Patent JPH0646862

Kind Code:

A

Abstract:

PURPOSE: To obtain a gene useful for producing cephalosporin C.

CONSTITUTION: A gene (cefG) encoding acetyl CoA: deacetylcephalosporin C acetyltransferase(DAC-ATF) of Cephalosporium acremonium has, for example, a nucleotide of the formula. C. acremonium C-10 is used as a DNA source, the gene library of C. acremonium C-10 is constructed on bleEMBL3 vector, a phage against cefEF gene is screened by probe K (5'- GGCAAGTACTCGGACTACTCGACGTGCTAC-3') to give a complete cefG gene positioned in a flanking DNA region of the cefEF gene and a complete gene constituted of 444 amino acids from BamHI fragment of 7.2 kb.

Inventors:

FUAN FURANSHISUKO MARUCHIN MAR
SANTEIAGO GUTEIERESU MARUCHIN
HABIERU BERASUKO ARUBARESU
FURANSHISUKO HOSE FUERUNANDESU

Application Number:

JP10503393A

Publication Date:

February 22, 1994

Filing Date:

April 07, 1993

Export Citation:

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Assignee:

HOECHST AG

International Classes:

C12N1/15; C12N15/09; C12N15/54; C12P35/06; C12R1/75; (IPC1-7): C12N15/54; C12N1/15; C12P35/06

Attorney, Agent or Firm:

Kazuo Sato (2 outside)

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