Title:
蛍光消光検出試薬及び方法
Document Type and Number:
Japanese Patent JP5019688
Kind Code:
B2
Abstract:
Oligonucleotide-fluorophore-quencher conjugates wherein the fluorophore moiety has emission wavelengths in the range of about (300) to about (800) nm, and or where the quencher includes a substituted 4-(phenyldiazenyl)phenylamine structure provide improved signal to noise ratios and other advantageous characteristics in hybridization and related assays. The oligonucleotide-fluorophore-quencher conjugates can be synthesized by utilizing novel phosphoramidite reagents that incorporate the quencher moiety based on the substituted 4-(phenyldiazenyl)phenylamine structure, and or novel phosphoramidite reagents that incorporate a fluorophore moiety based on the substituted coumarin, substituted 7-hydroxy-3H-phenoxazin-3-one, or substituted 5,10-dihydro-10 [phenyl]pyrido[2,3-d;6,5-d']dipyrimidine-2,4,6,8-(1H, 3H, 7H, 9H, 10H)-tetrone structure. Oligonucleotide-fluorophore-quencher-minor groove binder conjugates including a pyrrolo[4,5-e]indolin-7-yl}carbonyl)pyrrolo[4,5-e]indolin-7-yl]carbonyl}pyrrolo[4,5-e]indolin-7-caroxylate (DPI3) moiety as the minor groove binder and the substituted 4-(phenyldiazenyl)phenylamine moiety as the quencher, were synthesized and have substantially improved hybridization and signal to noise ratio properties.
Inventors:
Reed, Michael, W
Luktanov, Eugene, Alexander
Goal, alexander, a
Dempsey, Robert, Oh
Berousov, Jevgeny, Es
Luktanov, Eugene, Alexander
Goal, alexander, a
Dempsey, Robert, Oh
Berousov, Jevgeny, Es
Application Number:
JP2001544376A
Publication Date:
September 05, 2012
Filing Date:
December 08, 2000
Export Citation:
Assignee:
Epoch Biosciences Incorporated
International Classes:
C12N15/09; C07C245/08; C07D519/00; C07F9/24; C07F9/572; C07F9/655; C07F9/6561; C07H21/00; C07H21/04; C09B29/08; C12Q1/68; C07B61/00
Domestic Patent References:
| JPH07179773A | 1995-07-18 | |||
| JPH0578300A | 1993-03-30 | |||
| JPH04279556A | 1992-10-05 |
Foreign References:
| WO1997039008A1 | 1997-10-23 |
Attorney, Agent or Firm:
Shuichiro Kitamura
Ichiro Miyake
Takashi Ota
Saki Naoko Yama
Ichiro Miyake
Takashi Ota
Saki Naoko Yama