糖脂質アシル基転移酵素変異体及びその製造方法

Title:

糖脂質アシル基転移酵素変異体及びその製造方法

Document Type and Number:

Japanese Patent JP5697294

Kind Code:

B2

Abstract:

The present invention relates to a method of producing a variant glycolipid acyltransferase enzyme comprising: (a) selecting a parent enzyme which is a glycolipid acyltransferase enzyme characterised in that the enzyme comprises the amino acid sequence motif GDSX, wherein X is one or more of the following amino acid residues L, A, V, I, F, Y, H, Q, T, N, M or S; (b) modifying one or more amino acids to produce a variant glycolipid acyltransferase; (c) testing the variant glycolipid acyltransferase for transferase activity, an optionally hydrolytic activity, on a galactolipid substrate, and optionally a phospholipid substrate and/or optionally a triglyceride substrate; (d) selecting a variant enzyme with an enhanced activity towards galactolipids compared with the parent enzyme; and optionally (e) preparing a quantity of the variant enzyme. The present invention further relates to variant lipid acyltransferase enzyme characterised in that the enzyme comprises the amino acid sequence motif GDSX, wherein X is one or more of the following amino acid residues L, A, V, I, F, Y, H, Q, T, N, M or S, and wherein the variant enzyme comprises one or more amino acid modifications compared with a parent sequence at any one or more of the amino acid residues defined in set 2, set 4, set 6 or set 7.

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Inventors:

Kellet-Smith Annya Hemmingsen
Lorenzen Rige Hog
Zoe Youn Bosch
Michael Sen Young Dal Guard
Clay Arnode

Application Number:

JP2006546409A

Publication Date:

April 08, 2015

Filing Date:

December 23, 2004

Export Citation:

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Assignee:

DuPont Nutrition Biosciences ApS

International Classes:

C12N9/10; C12N15/54; A21D8/04; A23L1/30; C12N9/14; C12N11/00; C12N15/09; C12N15/55; C12P7/64; C12P13/00

Other References:

J.Bacteriol.,Vol.178,No.7(1996)p.2060-2064
JBC,Vol.269,No.3(1994)p.2146-2150

Attorney, Agent or Firm:

Masaki Hirota
Seiji Ozawa
Yusaku Tokai
Large High Toshiko
High Kazuya Tsu
Makoto Horiuchi

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