Title:
強化目的のフラックスに基づいたフラックス・スキャニングを用いた生物の改良方法
Document Type and Number:
Japanese Patent JP2008527992
Kind Code:
A
Abstract:
Provided is a method for improving useful substance-producing organisms using metabolic flux analysis, and more particularly a method for improving a host organism producing a useful substance, the method including: calculating a maximum flux value corresponding to the theoretical maximum yield of the useful substance in the metabolic network model of the host organism for producing useful substance, and calculating the optimum value of metabolic flux associated with useful substance production in the metabolic network when the value of cell growth-associated metabolic flux is the maximum under the condition where fermentation data are applied or not applied; selecting metabolic fluxes whose absolute values increase from the range between the maximum value and the optimum value; screening genes associated with the selected metabolic fluxes; and introducing and/or amplifying the selected genes in the host organism. Production of the useful substance can be effectively improved by using the method.
Inventors:
Issanyup
Wuhan Min
Choi Hyun Suk
Wuhan Min
Choi Hyun Suk
Application Number:
JP2007552049A
Publication Date:
July 31, 2008
Filing Date:
September 15, 2005
Export Citation:
Assignee:
ELECTRONICS AND TELECOMMUNICATIONS RESEARCH INSTITUTE
International Classes:
C12Q1/00; G16B5/00; C12N1/21; C12N15/09; C12P7/42; C12P17/16; C12P23/00
Domestic Patent References:
| JP2005058226A | 2005-03-10 | |||
| JP2005293394A | 2005-10-20 | |||
| JP2004520829A | 2004-07-15 | |||
| JP2005058226A | 2005-03-10 | |||
| JP2005293394A | 2005-10-20 | |||
| JP2004520829A | 2004-07-15 |
Attorney, Agent or Firm:
Kenichi Morita
Kenjiro Yamaguchi
Kenjiro Yamaguchi