Title:
A kit for transparency living thing specimen production, and a transparency living thing specimen manufacturing method
Document Type and Number:
Japanese Patent JP6274443
Kind Code:
B2
Abstract:
Provided are: a method and a kit for producing a biological specimen clear, which enable the clearing within a short time and by which a wide variety of biological specimens can be cleared; and a method and a kit for preserving a cleared biological specimen. A method for producing a cleared biological specimen, comprising steps as mentioned in items (A) to (C). (A) Step (1) of treating a material to be cleared with a fixing solution that is an aqueous solution containing paraformaldehyde or the like, a nonionic surfactant (at a concentration of 1% or more) and an alkali and optionally containing a buffering agent to produce the material that is cleared is involved. (B) Step (2) of treating the material that is produced in step (1) or a material to be cleared which is produced by a conventional fixing method with a clearing accelerator solution that is an aqueous solution containing a nonionic surfactant (at a concentration of 1% or more) and an alkali to produce the material that is cleared is involved. (C) Step (3) of treating the material produced in step (1) or (2) with a preservation solution that is an aqueous solution containing a nonionic surfactant (at a concentration of 1% or more) and a polyhydric alcohol to produce the material that is cleared is involved. A kit which can be used in the method.
Inventors:
Toshihisa Hatta
Mai Uchiba
Nobuaki Azuma
Hiroki Shimada
Eriko Shimamura
Mai Uchiba
Nobuaki Azuma
Hiroki Shimada
Eriko Shimamura
Application Number:
JP2014544550A
Publication Date:
February 07, 2018
Filing Date:
October 30, 2013
Export Citation:
Assignee:
Kanazawa Medical University
International Classes:
A01N1/02
Domestic Patent References:
| JP3167472A | ||||
| JP2005532824A | ||||
| JP2008239487A | ||||
| JP2004115482A |
Foreign References:
| WO2011111876A1 | ||||
| US6207408 | ||||
| WO2011020612A1 |
Other References:
「センセイ必見!!簡単DIYでアッと驚く魅せて教える科学実験9 排水口洗浄剤と染色試薬を使って透明骨格標本,化学,2013年 9月 1日,第68巻 第9号,p.46-49
Attorney, Agent or Firm:
Patent Service Corporation Patent Office Sykes