Title:
the living thing of temperature sensitivity -- the related method of collecting active biopolymers and cells
Document Type and Number:
Japanese Patent JP6125494
Kind Code:
B2
Abstract:
The present invention relates to a biopolymer and a cell-harvesting scaffold comprising same, as well as the associated cell-harvesting method that allows said harvesting to be performed in a simple and effective manner by reducing the culture temperature. The present invention also relates to a method for synthesising said biopolymer.
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Inventors:
Rodriguez-Cavelo, Jose Carlos
Arias Vallejo, Francisco Javier
Alonso Rodrigo, Matilde
Santos Garcia, Mercedes
Pierna Alvarez, Maria
Arias Vallejo, Francisco Javier
Alonso Rodrigo, Matilde
Santos Garcia, Mercedes
Pierna Alvarez, Maria
Application Number:
JP2014514116A
Publication Date:
May 10, 2017
Filing Date:
June 08, 2012
Export Citation:
Assignee:
UNIVERSIDAD DE VALLADOLID
International Classes:
C12N15/09; C07K14/00; C12M1/00; C12M3/00; C12N1/15; C12N1/19; C12N1/21; C12N5/00; C12N5/10; C12P21/00
Domestic Patent References:
| JP2010521246A | ||||
| JP2001340076A | ||||
| JP2007518811A |
Foreign References:
| WO2010092224A1 | ||||
| WO2007112048A2 |
Other References:
Zhang, H. et al.,"Human amniotic cell sheet harvest using a novel temperature-responsive culture surface coated with protein-based polymer",Tissue Eng.,2006年,Vol. 12,pp. 391-401
Costa, R. et al.,"Stimuli-Responsive Thin Coatings Using Elastin-Like Polymers for Biomedical Applications",Adv. Funct. Mater.,2009年,Vol. 19,pp. 3210-3218
Kurihara, H. et al.,"Recombinant extracellular matrix-like proteins with repetitive elastin or collagen-like functional motifs",Biotechnol. Lett.,2005年,Vol. 27,pp. 665-670
Kaufmann, D. et al.,"Chemical conjugation of linear and cyclic RGD moieties to a recombinant elastin-mimetic polypeptide - a versatile approach towards bioactive protein hydrogels",Macromol. Biosci.,2008年,Vol. 8,pp. 577-588
Ribeiro, A. et al.,"Influence of the amino-acid sequence on the inverse temperature transition of elastin-like polymers",Biophys. J.,2009年,Vol. 97,pp. 312-320
Luan C. et al.,"Solvent Deuteration Enhancement of Hydrophobicity: DSC Study of the Inverse Temperature Transition of Elastin-Based Polypeptides",J. Phys. Chem.,1991年,Vol. 95,pp. 7896-7900
Yamaoka, T. et al,"Mechanism for the phase transition of a genetically engineered elastin model peptide (VPGIG)40 in aqueous solution",Biomacromolecules,2003年,Vol. 4,pp. 1680-1685
Banta, S. et al.,"Protein engineering in the development of functional hydrogels",Annu. Rev. Biomed. Eng.,2010年,Vol. 12,pp. 167-186
Costa, R. et al.,"Stimuli-Responsive Thin Coatings Using Elastin-Like Polymers for Biomedical Applications",Adv. Funct. Mater.,2009年,Vol. 19,pp. 3210-3218
Kurihara, H. et al.,"Recombinant extracellular matrix-like proteins with repetitive elastin or collagen-like functional motifs",Biotechnol. Lett.,2005年,Vol. 27,pp. 665-670
Kaufmann, D. et al.,"Chemical conjugation of linear and cyclic RGD moieties to a recombinant elastin-mimetic polypeptide - a versatile approach towards bioactive protein hydrogels",Macromol. Biosci.,2008年,Vol. 8,pp. 577-588
Ribeiro, A. et al.,"Influence of the amino-acid sequence on the inverse temperature transition of elastin-like polymers",Biophys. J.,2009年,Vol. 97,pp. 312-320
Luan C. et al.,"Solvent Deuteration Enhancement of Hydrophobicity: DSC Study of the Inverse Temperature Transition of Elastin-Based Polypeptides",J. Phys. Chem.,1991年,Vol. 95,pp. 7896-7900
Yamaoka, T. et al,"Mechanism for the phase transition of a genetically engineered elastin model peptide (VPGIG)40 in aqueous solution",Biomacromolecules,2003年,Vol. 4,pp. 1680-1685
Banta, S. et al.,"Protein engineering in the development of functional hydrogels",Annu. Rev. Biomed. Eng.,2010年,Vol. 12,pp. 167-186
Attorney, Agent or Firm:
Harakenzo world patent & trademark