Title:
MANUFACTURING METHOD OF HIGH MOLECULAR WEIGHT SILK FIBROIN AQUEOUS SOLUTION, AND MANUFACTURING METHOD OF HIGH MOLECULAR WEIGHT SILK FIBROIN POWDER
Document Type and Number:
Japanese Patent JP2017119647
Kind Code:
A
Abstract:
PROBLEM TO BE SOLVED: To provide a manufacturing method of high molecular weight silk fibroin aqueous solution with excellent storage stability.SOLUTION: A manufacturing method of high molecular weight silk fibroin aqueous solution comprises: a neutral salt dissolution process of dissolving refined silk fibroin raw material into neutral salt aqueous solution to obtain silk fibroin/neutral salt aqueous solution; a desalting process of desalting the silk fibroin/neutral salt aqueous solution to obtain silk fibroin aqueous solution; a heating process of heating the silk fibroin aqueous solution at 65 to 110°C for 30 to 60 minutes; a cooling process of rapidly cooling silk fibroin aqueous solution heated in the heating process to 15°C or less; and a microfiltration process of filtering silk fibroin aqueous solution, which was cooled in the cooling process, by using a microfiltration membrane.SELECTED DRAWING: Figure 1
Inventors:
MATSUDA MICHIO
Application Number:
JP2015256847A
Publication Date:
July 06, 2017
Filing Date:
December 28, 2015
Export Citation:
Assignee:
MATSUDA SILK FARM CORP INC
International Classes:
C07K14/435; A23L2/38; A23L2/52; A23L33/17; A61K8/64; A61K47/42; C07K1/14
Domestic Patent References:
| JP2001057851A | 2001-03-06 | |||
| JP2002287495A | 2002-10-03 | |||
| JP2002284795A | 2002-10-03 | |||
| JPH11180999A | 1999-07-06 | |||
| JP2010013384A | 2010-01-21 |
Foreign References:
| WO2001042300A1 | 2001-06-14 |
Attorney, Agent or Firm:
Nozomi Watanabe
Haruko Sanwa
Hideaki Ito
Haruko Sanwa
Hideaki Ito