Title:
核酸ライブラリーの作製方法
Document Type and Number:
Japanese Patent JP5882234
Kind Code:
B2
Abstract:
A method of preparing a nucleic acid library in droplets in contact with oil, including: (a) blunt-ending nucleic acid fragments in a droplet in the oil to yield blunt-ended nucleic acid fragments; (b) phosphorylating the blunt-ended nucleic acid fragments in a droplet in the oil to yield phosphorylated nucleic acid fragments; coupling A-tails to the phosphorylated nucleic acid fragments in a droplet in the oil to yield A-tailed nucleic acid fragments; and (d) coupling nucleic acid adapters to the A-tailed nucleic acid fragments in a droplet in the oil to yield the nucleic acid library comprising adapter-ligated nucleic acid fragments.
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Inventors:
Michael Gee Pollack
Allen Eckhart
Arjun Sudarsan
Vijay Srinivasan
Jeremy Rouse
Uichon Yi
Nicholas Trotta
Rahul de Peshwarkar
Prasanna Swar
Gregory F Smith
Scott Norton
Peter Rinaltz
Allen Eckhart
Arjun Sudarsan
Vijay Srinivasan
Jeremy Rouse
Uichon Yi
Nicholas Trotta
Rahul de Peshwarkar
Prasanna Swar
Gregory F Smith
Scott Norton
Peter Rinaltz
Application Number:
JP2012555078A
Publication Date:
March 09, 2016
Filing Date:
February 22, 2011
Export Citation:
Assignee:
ADVANCED LIQUID LOGIC, INC.
International Classes:
C12N15/09; C12Q1/68
Domestic Patent References:
| JP2009502202A | ||||
| JP2007525151A |
Foreign References:
| WO2009032167A1 | ||||
| WO2008103900A1 |
Other References:
Nucleic Acids Research,2007年,Vol.35, No.11,e83
Nucleic Acids Research,2003年,Vol.31, No.19,e118
Nucleic Acids Research,2003年,Vol.31, No.19,e118
Attorney, Agent or Firm:
Kenji Sugimura
Hitomi Yuki
Hitomi Yuki
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