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Title:
METHOD FOR PRODUCING TRANSGENIC PLANT
Document Type and Number:
Japanese Patent JP2017205103
Kind Code:
A
Abstract:
PROBLEM TO BE SOLVED: To provide a method for transformation of a plant without the use of a callus or tissue piece in an in vitro culture system; a plant transformation method without introducing a selectable marker gene; and a method for producing a transformed plant having excellent reproducibility.SOLUTION: According to the present invention, there is provided a method for transformation of plants. The method comprises the steps of: coating at least one kind of nucleic acid on fine particles having a diameter of 0.3 μm or more and 0.9 μm or less; and striking the coated fine particles onto the shoot apexes of embryos of ripened seeds using a gene gun; growing the shoot apexes with the struck coated fine particles to obtain plant bodies; and selecting a transformed plant body from the plant bodies.SELECTED DRAWING: Figure 4

Inventors:
HAMADA HARUYASU
YANARAKU YOZO
MIKI RYUJI
TAOKA NAOAKI
IMAI RYOZO
Application Number:
JP2017096886A
Publication Date:
November 24, 2017
Filing Date:
May 15, 2017
Export Citation:
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Assignee:
KANEKA CORP
NAT AGRICULTURE & FOOD RES ORG
International Classes:
A01H5/10; C12N15/09
Domestic Patent References:
JPH10503374A1998-03-31
Foreign References:
WO2002052025A22002-07-04
WO2014144987A22014-09-18
Other References:
NATURE PROTOCOLS, 2008, VOL.3, PP.410-418, JPN6021006967, ISSN: 0004566332
PLANT CELL REPORTS, 1993, VOL.12, PP.165-169, JPN6021006970, ISSN: 0004566333
BIOTECHNOLOGY, 1995, VOL.13, PP.677-682, JPN6021006974, ISSN: 0004566334
北海道立製試集報, 1991, VOL.62, PP.69-77, JPN6021006976, ISSN: 0004566335
愛知農総試研報, 2003, VOL.35, PP.143-147, JPN6021006979, ISSN: 0004566336
PLANT CELL REP, 2008, VOL.27, PP.1509-1519, JPN6021006982, ISSN: 0004566337
"TUNGSTEN METAL POWDER|MICRON METALS INC", 2020.11.26, RETRIEVED ON 2021.2.22, RETRIEVED FROM THE IN, JPN6021006983, ISSN: 0004566331
Attorney, Agent or Firm:
Takuya Shindo
Nakamichi Yoshihiro
Kazuyuki Ohira