Title:
化合物又は製剤における造血プロスタグランジンDシンターゼの有力リガンドを置換する能力をアッセイする方法
Document Type and Number:
Japanese Patent JP2011522524
Kind Code:
A
Abstract:
An exemplary embodiment may be directed to a fluorescence polarization assay that screens compounds or agents for their affinity to hematopoietic prostaglandin D synthase (H-PGDS) based on their ability to displace a fluorophore-containing detection analyte bound to an enzyme comprising the primary amino acid sequence of H-PGDS. Another exemplary embodiment utilizes an enzyme having a maltose binding protein amino-acid sequence fused with an N-terminus of the enzyme.
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Inventors:
Palaccar, neisha
Johnson, Jeffrey Kay.
McGowan, Curry El.
Maxi, Kirk W.
Endless, Gregory W.
Johnson, Jeffrey Kay.
McGowan, Curry El.
Maxi, Kirk W.
Endless, Gregory W.
Application Number:
JP2011509643A
Publication Date:
August 04, 2011
Filing Date:
May 13, 2009
Export Citation:
Assignee:
CAYMAN CHEMICAL COMPANY
International Classes:
C12Q1/25; C12N9/00; G01N33/15; G01N33/50
Domestic Patent References:
| JP2006511789A | 2006-04-06 | |||
| JP2003522325A | 2003-07-22 |
Foreign References:
| WO2007041634A1 | 2007-04-12 |
Attorney, Agent or Firm:
Toshiyuki Maruyama
Takao Miyano
Nobuyasu Nishioka
Toshiya Nagatsuka
Takahiro Kutoku
Takao Miyano
Nobuyasu Nishioka
Toshiya Nagatsuka
Takahiro Kutoku