Title:
メチル化ＤＮＡを検出する方法、及びキット
Document Type and Number:
Japanese Patent JP5774805
Kind Code:
B2
Abstract:
The present invention relates to an in vitro method for detecting methylated DNA comprising (a) coating a container with a polypeptide capable of binding methylated DNA; (b) contacting said polypeptide with a sample comprising methylated and/or unmethylated DNA; and (c) detecting the binding of said polypeptide to methylated DNA. In a preferred embodiment, said method further comprises step (d) analyzing the detected methylated DNA by sequencing. Another aspect of the present invention is a kit for detecting methylated DNA according to the methods of the invention comprising (a) a polypeptide capable of binding methylated DNA; (b) a container which can be coated with said polypeptide; (c) means for coating said container; and (d) means for detecting methylated DNA.
Inventors:
Michael Leri
Application Number:
JP2007541868A
Publication Date:
September 09, 2015
Filing Date:
November 28, 2005
Export Citation:
Assignee:
Sequenom, Incorporated
International Classes:
C12Q1/68; C07K14/47; C12N15/09; G01N33/53; G01N33/543; G01N33/574
Domestic Patent References:
| JP2003125766A | ||||
| JP2008518596A |
Foreign References:
| WO2004065625A1 | ||||
| WO2002101353A1 |
Other References:
Nucleic Acids Res.,Vol.29,No.24(2001)p.e123
Attorney, Agent or Firm:
Atsushi Aoki
Takashi Ishida
Fukumoto product
Tetsuji Koga
Yoichi Watanabe
Ryotaro Takei
Takahide Sasaki
Takashi Ishida
Fukumoto product
Tetsuji Koga
Yoichi Watanabe
Ryotaro Takei
Takahide Sasaki