Title:
制限断片のクローン源を識別するための方法
Document Type and Number:
Japanese Patent JP5801349
Kind Code:
B2
Abstract:
The present invention relates to a high throughput method for the identification and detection of molecular markers wherein restriction fragments are generated and suitable adaptors comprising (sample-specific) identifiers are ligated. The adapter-ligated restriction fragments may be selectively amplified with adaptor compatible primers carrying selective nucleotides at their 3' end. The amplified adapter-ligated restriction fragments are, at least partly, sequenced using high throughput sequencing methods and the sequence parts of the restriction fragments together with the sample-specific identifiers serve as molecular markers.
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Inventors:
Van Ike, Michael Josephus Teresia
Jesse, Takopetel
Jesse, Takopetel
Application Number:
JP2013124744A
Publication Date:
October 28, 2015
Filing Date:
June 13, 2013
Export Citation:
Assignee:
Key Jean NAMROSE FENNOTE SHAP
International Classes:
C12N15/09; C12Q1/34; C12Q1/68; C40B20/04
Domestic Patent References:
| JP2003525019A | ||||
| JP2006516894A | ||||
| JP2008546405A |
Other References:
Genome Research,2000年,Vol.10,p.789-807
Theor Appl Genet,2006年 4月27日,Vol.113,p.81-89
Enphytica,2005年,Vol.141,p.129-137
Theor Appl Genet,2004年,Vol.108,p.1140-1146
Theor Appl Genet,2006年 4月27日,Vol.113,p.81-89
Enphytica,2005年,Vol.141,p.129-137
Theor Appl Genet,2004年,Vol.108,p.1140-1146
Attorney, Agent or Firm:
Kenho Ikeda
Shuichi Fukuda
Shuichi Fukuda