Title:
免疫グロブリン折りたたみ構造を持つタンパク質と、サブユニット構造となりうるタンパク質とを融合させた単量体タンパク質からなる多量体タンパク質の調製方法
Document Type and Number:
Japanese Patent JP5858251
Kind Code:
B2
Abstract:
The present invention provides a method for producing a multimeric protein consisting of a monomeric protein obtained by fusing a protein having an immunoglobulin fold structure to a protein that can serve as a subunit structure, the method including the steps of:
(A) preparing the monomeric protein having an insoluble granular form in cells of a microorganism;
(B) solubilizing the monomeric protein prepared in step (A) with an aqueous solution containing lauroyl-L-Glu;
(C) diluting a solution obtained in step (B) in a buffer containing arginine hydrochloride to lower a concentration of lauroyl-L-Glu; and
(D) replacing a solvent of a solution obtained in step (C) with a buffer using gel filtration chromatography or the like.
Inventors:
Daisuke Ejima
Haruna Sato
Kohei Tsumoto
Masayo Date
Haruna Sato
Kohei Tsumoto
Masayo Date
Application Number:
JP2013521655A
Publication Date:
February 10, 2016
Filing Date:
June 25, 2012
Export Citation:
Assignee:
AJINOMOTO CO.,LTD.
International Classes:
C12P21/02; C07K1/113; C07K1/16; C07K1/34; C07K1/36; C07K19/00
Foreign References:
| WO2009136568A1 |
Other References:
Protein Eng.,1996年,vol.9, no.2,pp.203-211
Bull. Korean Chem. Soc.,2009年,vol.30, no.5,pp.1101-1106
Bull. Korean Chem. Soc.,2009年,vol.30, no.5,pp.1101-1106
Attorney, Agent or Firm:
Koichi Tsujii
Sadao Kumakura
Atsushi Hakoda
Kenji Asai
Kazuo Yamazaki
Satsuki Ichikawa
Matsuda Nanae
Sadao Kumakura
Atsushi Hakoda
Kenji Asai
Kazuo Yamazaki
Satsuki Ichikawa
Matsuda Nanae