Title:
ヒト万能幹細胞から中胚葉幹細胞を生産する方法、及びその中胚葉幹細胞
Document Type and Number:
Japanese Patent JP5757434
Kind Code:
B2
Abstract:
Provided is a method for producing mesenchymal stem cells from human pluripotent stem cells, the method including: a) forming embryonic bodies from human pluripotent stem cells; b) attaching the embryonic bodies to a culture dish to induce natural differentiation of the embryonic bodies into mesenchymal stem cells; and c) performing continuous proliferative culturing of the mesenchymal stem cells while still maintaining the identity of the mesenchymal stem cells. Also, provided is a standardized method for inducing differentiation of mesenchymal stem cells, which can be broadly applied to all human pluripotent stem cells regardless of a difference in the genetic background thereof. Ultimately, the present invention can continuously mass-produces the mesenchymal stem cells necessary for regenerative medicine and cell therapy by using human pluripotent stem cells, thereby realizing practical uses of cell therapy products, and further the present invention is expected to highly contribute to treatments of incurable diseases, such as cardiovascular diseases and neurological disorders.
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Inventors:
Kim, Hyos
Kang, Hyunjae
Lee, Eunju
Park, Youngpae
Kang, Hyunjae
Lee, Eunju
Park, Youngpae
Application Number:
JP2012536618A
Publication Date:
July 29, 2015
Filing Date:
October 28, 2009
Export Citation:
Assignee:
SNU R&DB FOUNDATION
International Classes:
C12N5/0735; C12N5/0775
Foreign References:
| WO2009128533A1 | ||||
| WO2004106502A1 | ||||
| WO2003050250A1 | ||||
| WO2003004605A1 |
Other References:
TRIVEDI,P. AND HEMATTI,P.,Derivation of SSEA4-/CD73+ Mesenchymal Stem Cells from Human Embryonic Stem Cells.,Blood,2006年11月16日,Vol.108, No.11,p.729A,Abstract# 2579
OLIVIER,E.N. et al.,Differentiation of human embryonic stem cells into bipotent mesenchymal stem cells.,Stem Cells,2006年 8月,Vol.24, No.8,pp.1914-22
LEE,E.J. et al.,Novel embryoid body-based method to derive mesenchymal stem cells from human embryonic stem cells.,Tissue Eng. Part A,2010年 2月,Vol.16, No.2,pp.705-15
DANI,C. et al.,Differentiation of embryonic stem cells into adipocytes in vitro.,J. Cell Sci.,1997年 6月,Vol.110, No.11,pp.1279-85
ARPORNMAEKLONG,P. et al.,Phenotypic characterization, osteoblastic differentiation, and bone regeneration capacity of human embryonic stem cell-derived mesenchymal stem cells.,Stem Cells Dev.,2009年 9月,Vol.18, No.7,pp.955-68
Human Embryonic Stem (ES) Cell Embryoid Body Formation Medium.,MILLIPORE Catalog number SCM026,2007年12月,pp.1-3
OLIVIER,E.N. et al.,Differentiation of human embryonic stem cells into bipotent mesenchymal stem cells.,Stem Cells,2006年 8月,Vol.24, No.8,pp.1914-22
LEE,E.J. et al.,Novel embryoid body-based method to derive mesenchymal stem cells from human embryonic stem cells.,Tissue Eng. Part A,2010年 2月,Vol.16, No.2,pp.705-15
DANI,C. et al.,Differentiation of embryonic stem cells into adipocytes in vitro.,J. Cell Sci.,1997年 6月,Vol.110, No.11,pp.1279-85
ARPORNMAEKLONG,P. et al.,Phenotypic characterization, osteoblastic differentiation, and bone regeneration capacity of human embryonic stem cell-derived mesenchymal stem cells.,Stem Cells Dev.,2009年 9月,Vol.18, No.7,pp.955-68
Human Embryonic Stem (ES) Cell Embryoid Body Formation Medium.,MILLIPORE Catalog number SCM026,2007年12月,pp.1-3
Attorney, Agent or Firm:
Ryu Iwatani