Title:
アポトーシス調整化合物をスクリーニングする方法、前記方法により同定される化合物、および前記化合物の治療剤としての使用
Document Type and Number:
Japanese Patent JP4414231
Kind Code:
B2
Abstract:
The invention relates to a method of screening cellular polypeptides for pro-apoptotic or anti-apoptotic activity in a cell of a particular cell-type, said method comprising a. culturing cells of said particular cell-type under non apoptotic conditions and culturing cells of said particular cell-type under apoptotic conditions; and, b. determining subcellular localisation of said cellular polypeptides in the cultured cells; wherein a localization of a cellular polypeptide in lipid rafts in cultured cells under non apoptotic conditions and a segregation of said cellular polypeptide from lipid rafts in cultured cells under apoptotic conditions is indicative that said cellular polypeptide has a pro-apoptotic or an anti-apoptotic activity in said particular cell-type.
Inventors:
Garcia, Alphonse
Kaila, Xavier
Rebollo, Angelita
Iron, veronica
Flyer, arné
Kaila, Xavier
Rebollo, Angelita
Iron, veronica
Flyer, arné
Application Number:
JP2003573423A
Publication Date:
February 10, 2010
Filing Date:
March 07, 2003
Export Citation:
Assignee:
INSTITUT PASTEUR
CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS
Center National de la Recherche Scientific-Se en Ale S-
CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS
Center National de la Recherche Scientific-Se en Ale S-
International Classes:
C12Q1/02; A61K31/58; A61K31/724; A61K45/00; A61P9/10; A61P17/02; A61P25/28; A61P29/00; A61P31/00; A61P31/12; A61P31/18; A61P35/00; A61P37/02; A61P43/00; C07J21/00; C08B37/16; G01N33/15; G01N33/50; G01N33/68; C12R1/91
Foreign References:
| WO2000037088A1 |
Other References:
Blood, vol. 98, pages 3860-3863 (2001)
Biochem. Biophys. Res. Commun., vol. 287, pages 77-82 (2001)
J. Immunol., vol. 168, pages 3387-3393 (Apr. 2002)
Biochimie, vol. 85, pages 727-731 (2003)
Exp. Hematol., vol. 29, page 82, 322 (2001)
Eur. J. Immunol., vol. 31, pages 3789-3797 (2001)
Biochem. Biophys. Res. Commun., vol. 287, pages 77-82 (2001)
J. Immunol., vol. 168, pages 3387-3393 (Apr. 2002)
Biochimie, vol. 85, pages 727-731 (2003)
Exp. Hematol., vol. 29, page 82, 322 (2001)
Eur. J. Immunol., vol. 31, pages 3789-3797 (2001)
Attorney, Agent or Firm:
Takehiko Suzue
Satoshi Kono
Makoto Nakamura
Kurata Masatoshi
Takashi Mine
Yoshihiro Fukuhara
Sadao Muramatsu
Ryo Hashimoto
Satoshi Kono
Makoto Nakamura
Kurata Masatoshi
Takashi Mine
Yoshihiro Fukuhara
Sadao Muramatsu
Ryo Hashimoto