Title:
ピクテ・スペングラー反応を介して得られたタグ付けヌクレオシドを用いたシーケンシング反応のための方法
Document Type and Number:
Japanese Patent JP6977058
Kind Code:
B2
Abstract:
The present invention relates to improving the processing rate of a sequencing reaction, for example in a nanopore sequencing reaction, by means of using improved nucleoside-tags. The tags are linked to the nucleoside phosphate via a Pictet Spengler reaction. Exemplary sequencing reactions that are improved by the present methods include nanopore-based nucleic acid sequencing-by-synthesis reactions.
Inventors:
Frank Mang, Frank
Kuhelmeister, Hannes
Krisari, Peter
Peprun, Zebastian Johannes
Berner, Christian
Chow, Ian Kuang
Kaleward-Rum, Hanner Marie
Sakibu, Hassham
Kuhelmeister, Hannes
Krisari, Peter
Peprun, Zebastian Johannes
Berner, Christian
Chow, Ian Kuang
Kaleward-Rum, Hanner Marie
Sakibu, Hassham
Application Number:
JP2019555901A
Publication Date:
December 08, 2021
Filing Date:
April 11, 2018
Export Citation:
Assignee:
F. HOFFMANN-LA ROCHE AKTIENGESELLSCHAFT
International Classes:
C12Q1/6869; C07H21/04; C12Q1/6806
Other References:
WU, F., et al.,"Scaffold tailoring by a newly detected Pictet-Spenglerase activity of strictosidine synthase: from the common tryptoline skeleton to the rare piperazino-indole framework.",J. AM. CHEM. SOC.,2012年01月25日,Vol.134, No.3,pp.1498-1500,doi: 10.1021/ja211524d. Epub 2012 Jan 9
Attorney, Agent or Firm:
Shinjiro Ono
Osamu Yamamoto
Toru Miyamae
Motoharu Nakanishi
Ichinomiya
Osamu Yamamoto
Toru Miyamae
Motoharu Nakanishi
Ichinomiya
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