Title:
脂質を菌体外に分泌する微生物ならびに当該微生物を用いた脂質および脂質を内包する油滴小胞の製造方法
Document Type and Number:
Japanese Patent JP4748907
Kind Code:
B2
Abstract:
There are provided microorganisms having a property of producing a lipid containing unsaturated fatty acids as constituent fatty acids and extracellularly secreting the produced lipid encapsulated in lipid vesicles, methods of screening said microorganisms, as well as methods of efficiently producing a fatty acid-containing lipid using said microorganisms. Furthermore, there are provided lipid vesicles encapsulating a lipid containing unsaturated fatty acids, and foods, cosmetics, and animal feeds comprising said lipid vesicles added thereto. Artificially treated microorganisms or microorganisms collected from nature are grown on a solid medium, and microbial strains that form lipid vesicles at the periphery of the colonies and/or microbial strains that, when cultured in a transparent liquid medium, make the culture liquid cloudy are selected. The microorganisms obtained are cultured, lipid-containing lipid vesicles secreted in the culture liquid, are separated from the culture liquid, and the lipid is separated and purified.
Inventors:
Kengo Akimoto
Hiroshi Kawashima
Sakayu Shimizu
Hiroshi Kawashima
Sakayu Shimizu
Application Number:
JP2001517665A
Publication Date:
August 17, 2011
Filing Date:
August 11, 2000
Export Citation:
Assignee:
Suntory Holdings Limited
International Classes:
C12N1/14; A23C9/152; A23C11/04; A23D7/00; A23D9/00; A23L2/52; A61K8/14; A61K8/92; A61K8/96; A61Q19/00; C12P7/64; C12Q1/02; C12R1/645
Domestic Patent References:
| JPH1057085A | 1998-03-03 | |||
| JPH0889167A | 1996-04-09 | |||
| JPH08163990A | 1996-06-25 | |||
| JPS63240791A | 1988-10-06 | |||
| JPS623791A | 1987-01-09 |
Foreign References:
| WO1998039468A1 | 1998-09-11 |
Attorney, Agent or Firm:
Atsushi Aoki
Takashi Ishida
Fukumoto product
Tetsuji Koga
Kazuhiro Nakamura
Yoichi Watanabe
Masaru Nakajima
Takashi Ishida
Fukumoto product
Tetsuji Koga
Kazuhiro Nakamura
Yoichi Watanabe
Masaru Nakajima